Transfection — is the process of introducing nucleic acids into cells by non viral methods [http://www.promega.com/guides/transfxn guide/transfxn.pdf] . The term transformation is preferred to describe non viral DNA transfer in bacteria and non animal… … Wikipedia
transient expression — (= transient transfection) When transfecting animal cells, cells in which the transgene has not been physically incorporated into the genome (stable transfection), but is carried as an episome that can be lost. This means that expression levels… … Dictionary of molecular biology
Optical transfection — is the process of introducing nucleic acids into cells using light. Typically, a laser is focussed to a diffraction limited spot ( 1 µm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then… … Wikipedia
Molecular biology — (pronounced /məˈlɛkjʊlər .../) is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology… … Wikipedia
List of molecular biology topics — This is a list of topics in molecular biology. See also list of biochemistry topics. NOTOC # 3 end 3 flanking region 5 end 5 flanking region 5 ribose 3 A acrylamide gels adenine adenosine deaminase deficiency adenovirus agarose gel… … Wikipedia
Diethylaminoethyl cellulose — Schematic structure of DEAE: positively charged DEAE groups can bind negative ions Diethylaminoethyl cellulose (DEAE or DEAE C) is a positively charged resin used in ion exchange chromatography, a type of column chromatography, used in protein… … Wikipedia
VA RNA — The VA (viral associated) RNA is a non coding RNA found in adenovirus. [cite journal | last = Mathews | first = MB | year = 1995 | title = Structure, function, and evolution of adenovirus virus associated RNAs | journal = Curr Top Microbiol… … Wikipedia
DamID — DamID[1] (DNA adenine methyltransferase identification) is a molecular biology protocol used to map the binding sites of DNA and chromatin binding proteins in eukaryotes. DamID identifies binding sites by expressing the proposed DNA binding… … Wikipedia
Gene therapy — using an Adenovirus vector. A new gene is inserted into an adenovirull. If the treatment is successful, the new gene will make a functional protein. Gene therapy is the insertion, alteration, or removal of genes within an individual s cells and… … Wikipedia
endocrine system, human — ▪ anatomy Introduction group of ductless glands (gland) that regulate body processes by secreting chemical substances called hormones (hormone). Hormones act on nearby tissues or are carried in the bloodstream to act on specific target organs… … Universalium
Transformation (genetics) — An unrelated process called malignant transformation occurs in the progression of cancer. In molecular biology transformation is the genetic alteration of a cell resulting from the direct uptake, incorporation and expression of exogenous genetic… … Wikipedia